Publications:
A new semi-automated plug-based crystallization technology:
"The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS)", Gerdts, C. J., Elliot, M., Lovell, S., Mixon, M. B., Napuli, A. J., Staker, B.L., Nollert, P., & Stewart, L. (2008) Acta Cryst. D64, 1116-1122.
An introduction to plug-based technology:
"A Microfluidic System for Controlling Reaction Networks in Time," Song, H., Tice, J. D. & Ismagilov, R. F. (2003). Angew. Chem. Int. Ed. 7, 768-772.
Fundamentals of microfluidic plug-based technology:
"Formation of Droplets and Mixing in Multiphase Microfludics at Low Values of the Reynolds and the Capillary Numbers"
Tice,J. D., Song, H., Lyon, A. D., & Ismagilov, R. F. (2003) Langmuir. 19, 9127-9133.
In introduction to protein crystallization in plugs:
“Screening of Protein Crystallization Conditions on a Microfluidic Chip Using Nanoliter-Size Droplets"
Zheng, B., Roach, L. S. & Ismagilov, R. F. (2003). J. Am. Chem. Soc. 11170-11171.
A review of plug-based protein crystallization studies:
"Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization"
Zheng, B., Gerdts, C. J. & Ismagilov, R. F. (2005). Curr. Op. Struc. Biol. 548-555.
Sparse matrix screening and membrane protein crystallization in plugs:
"Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins"
Li, L., Mustafi, D., Fu, Q., Tereshko, V., Chen, D. L., Tice, J. D. & Ismagilov, R. F. (2006). PNAS.
Plug-based protein crystallization combined with in situ diffraction:
“In situ data collection and structure refinement from microcapillary protein crystallization”
Yadav, M. K., Gerdts, C. J., Sanishvili, R., Smith, W. W., Roach, L. S., Ismagilov, R. F., Kuhn, P. & Stevens, R. C. (2005). J. Appl. Cryst. 900-905.
Microfluidic seeding using plug-based protein crystallization:
"Time-Controlled Microfluidic Seeding in nL-Volume Droplets To Separate Nucleation and Growth Stages of Protein Crystallization"
Gerdts, C. J., Tereshko, V., Yadav, M. K., Demetieva, I., Collart, F., Joahchimiak, A., Stevens, R. C., Kuhn, P., Kossiakoff, A. & Ismagilov, R. F. (2006). Angew. Chem. Int. Ed. 8456-8160.
